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Published online March 24, 2008
doi:10.1083/jcb.200708170
The Journal of Cell Biology, Vol. 180, No. 6, 1115-1131
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Nakazawa et al.
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Article

 Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis

Norihiko Nakazawa1, Takahiro Nakamura1, Aya Kokubu2, Masahiro Ebe1, Koji Nagao2, and Mitsuhiro Yanagida1,2

1 Core Research for Evolutional Science and Technology Research Program, Japan Science and Technology Corporation, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
2 G0 Cell Unit, Okinawa Institute of Science and Technology Promotion Corporation, Uruma, Okinawa 904-2234, Japan

Correspondence to Mitsuhiro Yanagida: yanagida{at}kozo.lif.kyoto-u.ac.jp

The condensin complex has a fundamental role in chromosome dynamics. In this study, we report that accumulation of Schizosaccharomyces pombe condensin at mitotic kinetochores and ribosomal DNAs (rDNAs) occurs in multiple steps and is necessary for normal segregation of the sister kinetochores and rDNAs. Nuclear entry of condensin at the onset of mitosis requires Cut15/importin {alpha} and Cdc2 phosphorylation. Ark1/aurora and Cut17/Bir1/survivin are needed to dock the condensin at both the kinetochores and rDNAs. Furthermore, proteins that are necessary to form the chromatin architecture of the kinetochores (Mis6, Cnp1, and Mis13) and rDNAs (Nuc1 and Acr1) are required for condensin to accumulate specifically at these sites. Acr1 (accumulation of condensin at rDNA 1) is an rDNA upstream sequence binding protein that physically interacts with Rrn5, Rrn11, Rrn7, and Spp27 and is required for the proper accumulation of Nuc1 at rDNAs. The mechanism of condensin accumulation at the kinetochores may be conserved, as human condensin II fails to accumulate at kinetochores in hMis6 RNA interference–treated cells.

Abbreviations used in this paper: ARS, autonomously replicating sequence; CENP, centromere protein; ChIP, chromatin immunoprecipitation; mCherry, monomeric Cherry; NTS, nontranscribing sequence; rDNA, ribosomal DNA; SMC, structural maintenance of chromosome; SPB, spindle pole body; ts, temperature sensitive.


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