JCB logo
Free T-shirt with ExoSAP-IT purchase.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online April 14, 2008
doi:10.1083/jcb.200711105
The Journal of Cell Biology, Vol. 181, No. 2, 195-202
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Hu et al.
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Hu, C.-K.
Right arrow Articles by Mitchison, T. J.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hu, C.-K.
Right arrow Articles by Mitchison, T. J.
Related Collections
Right arrowRelated In this Issue article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Report

 Cell polarization during monopolar cytokinesis

Chi-Kuo Hu1,2, Margaret Coughlin1, Christine M. Field1, and Timothy J. Mitchison1

1 Department of Systems Biology and 2 Graduate Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 02115

Correspondence to Chi-Kuo Hu: ckhu{at}fas.harvard.edu

During cytokinesis, a specialized set of proteins is recruited to the equatorial region between spindle poles by microtubules and actin filaments, enabling furrow assembly and ingression before cell division. We investigate the mechanisms underlying regional specialization of the cytoskeleton in HeLa cells undergoing drug-synchronized monopolar cytokinesis. After forced mitotic exit, the cytoskeleton of monopolar mitotic cells is initially radially symmetric but undergoes a symmetry-breaking reaction that simultaneously polarizes microtubules and the cell cortex, with a concentration of cortical furrow markers into a cap at one side of the cell. Polarization requires microtubules, F-actin, RhoA, Myosin II activity, and Aurora B kinase activity. Aurora B localizes to actin cables in a gap between the monopolar midzone and the furrow-like cortex, suggesting a communication between them. We propose that feedback loops between cortical furrow components and microtubules promote symmetry breaking during monopolar cytokinesis and regional specialization of the cytoskeleton during normal bipolar cytokinesis.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related In this Issue article

Polarizing for furrows
Nicole LeBrasseur
J. Cell Biol. 2008 181: 174. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents