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Published online April 28, 2008
doi:10.1083/jcb.200708082
The Journal of Cell Biology, Vol. 181, No. 3, 431-438
The Rockefeller University Press, 0021-9525 $30.00
© 2008 de Graffenried et al.
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 Polo-like kinase is required for Golgi and bilobe biogenesis in Trypanosoma brucei

Christopher L. de Graffenried1,2, Helen H. Ho1, and Graham Warren1,2

1 Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520
2 Max F. Perutz Laboratories, University of Vienna, Medical University of Vienna, Vienna 1030, Austria

Correspondence to C.L. de Graffenried: chris.degraffenried{at}univie.ac.at

A bilobed structure marked by TbCentrin2 regulates Golgi duplication in the protozoan parasite Trypanosoma brucei. This structure must itself duplicate during the cell cycle for Golgi inheritance to proceed normally. We show here that duplication of the bilobed structure is dependent on the single polo-like kinase (PLK) homologue in T. brucei (TbPLK). Depletion of TbPLK leads to malformed bilobed structures, which is consistent with an inhibition of duplication and an increase in the number of dispersed Golgi structures with associated endoplasmic reticulum exit sites. These data suggest that the bilobe may act as a scaffold for the controlled assembly of the duplicating Golgi.

Abbreviations used in this paper: ERES, ER exit site; FAZ, flagellum attachment zone; PLK, polo-like kinase.


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