JCB logo
Free T-shirt with ExoSAP-IT purchase.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online May 5, 2008
doi:10.1083/jcb.200710127
The Journal of Cell Biology, Vol. 181, No. 3, 475-483
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Wu et al.
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Wu, J.
Right arrow Articles by Wang, Y.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, J.
Right arrow Articles by Wang, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 Cdc14B depletion leads to centriole amplification, and its overexpression prevents unscheduled centriole duplication

Jun Wu1, Hyekyung P. Cho1, David B. Rhee1,3, Dabney K. Johnson1, John Dunlap2, Yie Liu3, and Yisong Wang1

1 Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831
2 Microscopy Facility, Division of Biology, University of Tennessee, Knoxville, TN 37996
3 Laboratory of Molecular Gerontology, Gerontology Research Center, National Institute on Aging, Baltimore, MD 21224

Correspondence to Y. Wang: ywa{at}ornl.gov; or Y. Liu: liuyie{at}grc.nia.nih.gov

Centrosome duplication is tightly controlled in coordination with DNA replication. The molecular mechanism of centrosome duplication remains unclear. Previous studies found that a fraction of human proline-directed phosphatase Cdc14B associates with centrosomes. However, Cdc14B's involvement in centrosome cycle control has never been explored. Here, we show that depletion of Cdc14B by RNA interference leads to centriole amplification in both HeLa and normal human fibroblast BJ and MRC-5 cells. Induction of Cdc14B expression through a regulatable promoter significantly attenuates centriole amplification in prolonged S phase–arrested cells and proteasome inhibitor Z-L3VS–treated cells. This inhibitory function requires centriole-associated Cdc14B catalytic activity. Together, these results suggest a potential function for Cdc14B phosphatase in maintaining the fidelity of centrosome duplication cycle.

J. Wu and H.P. Cho contributed equally to this paper.

Abbreviations used in this paper: DOX, doxycycline; HU, hydroxyurea; shRNA, small hairpin RNA.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents