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Published online May 12, 2008
doi:10.1083/jcb.200711160
The Journal of Cell Biology, Vol. 181, No. 4, 595-603
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Durcan et al.
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 Tektin 2 is required for central spindle microtubule organization and the completion of cytokinesis

Thomas M. Durcan, Elizabeth S. Halpin, Trisha Rao, Nicholas S. Collins, Emily K. Tribble, Jessica E. Hornick, and Edward H. Hinchcliffe

Department of Biological Sciences and Notre Dame Integrated Imaging Facility University of Notre Dame Notre Dame IN 46556

Correspondence to Edward H. Hinchcliffe: Hinchcliffe.3{at}nd.edu

During anaphase, the nonkinetochore microtubules in the spindle midzone become compacted into the central spindle, a structure which is required to both initiate and complete cytokinesis. We show that Tektin 2 (Tek2) associates with the spindle poles throughout mitosis, organizes the spindle midzone microtubules during anaphase, and assembles into the midbody matrix surrounding the compacted midzone microtubules during cytokinesis. Tek2 small interfering RNA (siRNA) disrupts central spindle organization and proper localization of MKLP1, PRC1, and Aurora B to the midzone and prevents the formation of a midbody matrix. Video microscopy revealed that loss of Tek2 results in binucleate cell formation by aberrant fusion of daughter cells after cytokinesis. Although a myosin II inhibitor, blebbistatin, prevents actin-myosin contractility, the microtubules of the central spindle are compacted. Strikingly, Tek2 siRNA abolishes this actin-myosin–independent midzone microtubule compaction. Thus, Tek2-dependent organization of the central spindle during anaphase is essential for proper midbody formation and the segregation of daughter cells after cytokinesis.

T.M. Durcan and E.S. Halpin contributed equally to this paper.

T.M. Durcan's present address is Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada H3A 2B4.

T. Rao's present address is Dept. of Biochemistry, McGill University, Montreal, Quebec, Canada H3G 1V6.

N.S. Collins' present address is Program in Genetic Counseling, University of Arkansas at Little Rock, Little Rock, AR 72204.

E.K. Tribble's present address is Dept. of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC 27599.

Abbreviations used in this paper: KD, knockdown; SCR, scrambled; UTR, untranslated region.


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