JCB logo
Keystone Symposia
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online May 12, 2008
doi:10.1083/jcb.200711165
The Journal of Cell Biology, Vol. 181, No. 4, 683-695
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Shen et al.
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Shen, L.
Right arrow Articles by Turner, J. R.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shen, L.
Right arrow Articles by Turner, J. R.
Related Collections
Right arrowRelated In this Issue article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 The tight junction protein complex undergoes rapid and continuous molecular remodeling at steady state

Le Shen, Christopher R. Weber, and Jerrold R. Turner

Department of Pathology, The University of Chicago, Chicago, IL 60637

Correspondence to Jerrold R. Turner: jturner{at}bsd.uchicago.edu

The tight junction defines epithelial organization. Structurally, the tight junction is comprised of transmembrane and membrane-associated proteins that are thought to assemble into stable complexes to determine function. In this study, we measure tight junction protein dynamics in live confluent Madin–Darby canine kidney monolayers using fluorescence recovery after photobleaching and related methods. Mathematical modeling shows that the majority of claudin-1 (76 ± 5%) is stably localized at the tight junction. In contrast, the majority of occludin (71 ± 3%) diffuses rapidly within the tight junction with a diffusion constant of 0.011 µm2s–1. Zonula occludens-1 molecules are also highly dynamic in this region, but, rather than diffusing within the plane of the membrane, 69 ± 5% exchange between membrane and intracellular pools in an energy-dependent manner. These data demonstrate that the tight junction undergoes constant remodeling and suggest that this dynamic behavior may contribute to tight junction assembly and regulation.

Abbreviations used in this paper: FLIP, fluorescent loss in photobleaching; MBCD, methyl-β-cyclodextrin; PA, photoactivable; TER. transepithelial resistance; ZO, zonula occludens.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related In this Issue article

Tight junctions loosen up
Mitch Leslie
J. Cell Biol. 2008 181: 568. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents