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The Journal of Cell Biology, Vol 19, 71-77, Copyright © 1963 by Rockefeller University Press

ARTICLE

UPTAKE OF MAMMALIAN CHROMOSOMES BY MAMMALIAN CELLS

M. Chorazdoty M.D.1, A. Bendich Ph.D.1, E. Borenfreund Ph.D.1, O. L. Ittensohn 1, and D. J. Hutchison Ph.D.1

1 From the Division of Experimental Chemotherapy and Division of Nucleoprotein Chemistry, Sloan-Kettering Institute for Cancer Research, and the Sloan-Kettering Division, Graduate School of Medical Sciences, Cornell University Medical College, New York.

Dr. Chorazdoty's present address is the Department of Tumor Biology, Institute of Oncology, Gliwice, Poland

Chromosomes isolated from mouse leukemia L1210 cells were taken up by mouse macrophages, HeLa cells, and rat embryo fibroblasts following simple exposure in vitro. The process, which resembles pinocytosis or phagocytosis, was traced by autoradiography of chromosomes prelabeled with thymidine-H3, and by staining techniques and phase contrast microscopy. During the first six hours, the uptake of chromosomes was restricted to the cytoplasm, but there was some evidence of penetration into the nucleus after 16 and 26 hours of exposure. Treatment of rat fibroblasts with glucose and insulin markedly enhanced the uptake of chromosomes, whereas iodoacetate inhibited their penetration.

Submitted on March 8, 1963


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