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The Journal of Cell Biology, Vol 24, 259-266, Copyright © 1965 by Rockefeller University Press

ARTICLE

LYSOSOMAL FRACTIONS FROM TRANSITIONAL EPITHELIUM

Norbert M. Kanczak Ph.D.1, Joseph I. Krall 1, E. Russell Hayes Ph.D.1, and Willard B. Elliott Ph.D.1

1 From the Departments of Anatomy and Biochemistry, State University of New York at Buffalo, School of Medicine, Buffalo.

Dr. Kanczak's present address is The Laboratory of Cellular Pathology, The Ohio State University, College of Medicine, Columbus

Histochemical data suggested that the so called lipoid granules of transitional epithelium in some species are equivalent to lysosomes. Scrapings of bovine and canine transitional epithelium were subjected to differential centrifugation to confirm this identification biochemically. Fractions of rat liver, the classic source of lysosomes, were also prepared by the same methods to compare with the fractions obtained from urinary epithelium. In contrast to rat liver, uroepithelial fractions with a high relative specific activity for hydrolases were sedimented before the heavy mitochondria. Microscopically, these fractions contained the highest proportion of lipoid granules. The size and sedimentation characteristics of lysosomes from transitional epithelium more closely resembled those of lysosomes derived from rat kidney than those isolated from liver.

Submitted on March 10, 1964


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