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The Journal of Cell Biology, Vol 24, 285-295, Copyright © 1965 by Rockefeller University Press

ARTICLE

LOCALIZATION OF THE SUCCINIC DEHYDROGENASE SYSTEM IN ESCHERICHIA COLI USING COMBINED TECHNIQUES OF CYTOCHEMISTRY AND ELECTRON MICROSCOPY

Albert W. Sedar Ph.D.1 and Ronald M. Burde M.D.1

1 From the Daniel Baugh Institute of Anatomy, Jefferson Medical College, Philadelphia

The activity of the succinic dehydrogenase system was studied in Escherichia coli utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evidenced by the deposition of aggregates of TNBT-formazan, was found associated with the site of the plasma membrane of the bacterium.

Submitted on March 24, 1964


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