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The Journal of Cell Biology, Vol 25, 113-121, Copyright © 1965 by Rockefeller University Press

ARTICLE

RESPIRATION OF RAT PERITONEAL MAST CELLS

Nirmal Chakravarty M.D.1 and Erik Zeuthen Ph.D.1

1 From the Biological Institute of the Carlsberg Foundation, Copenhagen, Denmark.

Dr. Chakravarty's present address is Department of Pharmacology, University of Umeå, Umeå Sweden

Methods for microgasometry of a few hundred mast cells are described. The Cartesian ampulla diver technique is used. The sample size is determined by counting the cells within the diver. The respiration rates at 37°C, expressed in microliters per cell per hour, are 0.29 x 10-6 without substrate and 0.47 x 10-6 with glucose.

Submitted on June 17, 1964


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