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The Journal of Cell Biology, Vol 25, 171-177, Copyright © 1965 by Rockefeller University Press

ARTICLE

H3-THYMIDINE DERIVATIVE POOLS IN RELATION TO MACRONUCLEAR DNA SYNTHESIS IN TETRAHYMENA PYRIFORMIS

G. E. Stone Ph.D.1, O. L. Miller Jr. Ph.D.1, and D. M. Prescott Ph.D.1

1 From the Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee.

The present address of Dr. Stone and Dr. Prescott is Department of Anatomy, University of Colorado Medical Center, Denver

The formation of a soluble H3-thymidine derivative pool has been examined in Tetrahymena pyriformis as a function of macronuclear DNA synthesis during the cell life cycle. An autoradiographic technique which allows the detection of water-soluble materials within a cell has shown that these cells do not take up and retain exogenous H3-thymidine during G1 or G2. Uptake of H3-thymidine is restricted to the S period of the cell cycle. Additional autoradiographic experiments show, however, that a soluble pool of H3-thymidine derivatives persists from the end of one DNA synthesis period to the beginning of the next synthesis period in the subsequent cell cycle. Since this persisting pool cannot be labeled with H3-thymidine, the pool does not turn over during non-S periods.

Submitted on July 10, 1964


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