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CORRELATED MORPHOLOGICAL AND PHYSIOLOGICAL STUDIES ON ISOLATED SINGLE MUSCLE FIBERS : I. Fine Structure of the Crayfish Muscle Fiber

Philip W. Brandt ¹, John P. Reuben ¹, Lucien Girardier ¹, and Harry Grundfest ¹

¹ From the Department of Anatomy and The Laboratory of Neurophysiology, Department of Neurology, College of Physicians and Surgeons, Columbia University, New York, and the Marine Biological Laboratory, Woods Hole, Massachusetts.

Dr. Girardier's present address is Institute of Physiology, University of Geneva, Geneva, Switzerland

Single fibers isolated from walking leg muscles of crayfish have 8- to 10-µ sarcomeres which are divided into A, I, and Z bands. The H zone is poorly defined and no M band is distinguishable. Changes in the width of the I band, accompanied by change in the overlap between thick and thin myofilaments, occur when the length of the sarcomere is changed by stretching or by shortening the fiber. The thick myofilaments (ca. 200 A in diameter) are confined to the A band. The thin myofilaments (ca. 50 A in diameter) are difficult to resolve except in swollen fibers, when they clearly lie between the thick filaments and run to the Z disc. The sarcolemma invaginates at 50 to 200 sites in each sarcomere. The sarcolemmal invaginations (SI) form tubes about 0.2 µ in diameter which run radially into the fiber and have longitudinal side branches. Tubules about 150 A in diameter arise from the SI and from the sarcolemma. The invaginations and tubules are all derived from and are continuous with the plasma membrane, forming the transverse tubular system (TTS), which is analogous with the T system of vertebrate muscle. In the A band region each myofibril is enveloped by a fenestrated membranous covering of sarcoplasmic reticulum (SR). Sacculations of the SR extend over the A-I junctions of the myofibrils, where they make specialized contacts (diads) with the TTS. At the diads the opposing membranes of the TTS and SR are spaced 150 A apart, with a 35-A plate centrally located in the gap. It appears likely that the anion-permselective membrane of the TTS which was described previously is located at the diads, and that this property of the diadic structures therefore may function in excitation-contraction coupling.

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This article has been cited by other articles:

• MILLMAN, B. M. (1998). The Filament Lattice of Striated Muscle. Physiol. Rev. 78: 359-391 [Abstract] [Full Text]  
• Ozeki, M. (1969). Crayfish Muscle Fiber: Spike Electrogenesis in Fibers with Long Sarcomeres. Science 163: 82-83 [Abstract]  

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