ARTICLE

COMBINED BIOCHEMICAL AND MORPHOLOGICAL STUDY OF PARTICULATE FRACTIONS FROM RAT LIVER : Analysis of Preparations Enriched in Lysosomes or in Particles Containing Urate Oxidase, D-Amino Acid Oxidase, and Catalase

Pierre Baudhuin M.D.¹, Henri Beaufay M.D.¹, and Christian de Duve M.D.¹

¹ From the Laboratory of Physiological Chemistry, University of Louvain, Belgium

Six particulate preparations isolated from rat liver under different experimental conditions were analyzed biochemically and examined in the electron microscope. The results confirm the lysosomal nature of the pericanalicular dense bodies and demonstrate that the microbodies are the bearers of urate oxidase, catalase, and D-amino acid oxidase. Catalase, representing a major component of the particles, and D-amino acid oxidase appear to be associated with the structureless "sap" of the particles, urate oxidase with their crystalloid core or with their outer membrane.

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