ARTICLE

THE NUCLEOLI IN MITOTIC DIVISIONS OF MAMMALIAN CELLS IN VITRO

T. C. Hsu ¹, Frances E. Arrighi ¹, Robert R. Klevecz ¹, and B. R. Brinkley ¹

¹ From the Section of Cytology, Department of Biology, The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas

In a number of mammalian cell strains nucleoli persisted through mitosis. This phenomenon was especially pronounced in several cell lines derived from Chinese hamster tissues. All the methods employed, including radioautography with tritiated uridine, cytochemical stains (methyl green-pyronin and azure B), fluorescent microscopy (coriphosphine O), ribonuclease digestion, and electron microscopy, demonstrated that the bodies identified as persistent nucleoli in the mitotic stages had the same characteristics as did the nucleoli in the interphase. Persistent nucleoli may attach to the chromosomes or may be free in the cytoplasm. In cells where no persistent nucleoli as such were noted, nucleolar material was observed to attach to the chromosomes in shapeless masses which moved with the chromosomes during anaphase. At least a portion of the nucleolar material was included in the daughter nuclei, presumably for immediate use for protein synthesis after cell division.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Dundr, M, Leno, G., Lewis, N, Rekosh, D, Hammarskjoid, M., Olson, M. (1996). Location of the HIV-1 Rev protein during mitosis: inactivation of the nuclear export signal alters the pathway for postmitotic reentry into nucleoli. J. Cell Sci. 109: 2239-2251 [Abstract]  
• Steward, D. L., Shaeffer, J. R., Humphrey, R. M. (1968). Breakdown and Assembly of Polyribosomes in Synchronized Chinese Hamster Cells. Science 161: 791-793 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents