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The Journal of Cell Biology, Vol 27, 119-132, Copyright © 1965 by Rockefeller University Press

ARTICLE

METHOD FOR COMBINED ULTRASTRUCTURAL AND BIOCHEMICAL ANALYSIS OF NEURAL TISSUE

W. Arnoldo Levy 1, Ivan Herzog 1, Kunihiko Suzuki 1, Robert Katzman 1, and Labe Scheinberg 1

1 From the Saul R. Korey Department of Neurology, and the Departments of Neurological Surgery and Pathology (Neuropathology), Albert Einstein College of Medicine of Yeshiva University, New York

No significant change was found in the electrolytes and lipids of the brain analyzed after glutaraldehyde fixation by perfusion of laboratory animals; such fixation also satisfactorily preserves neural tissues for electron microscopy. The brains of normal and tumor-bearing C3H mice, Wistar rats, and New Zealand rabbits were studied. Little difference was found in the dry weight and the content of sodium, potassium, total lipid and lipid fractions, and in the sulfate space (S35O4) between specimens from unperfused and perfused animals, whether normal or tumor-bearing. The results suggest the possibility of using selected regions of the nervous system, dissected after fixation, for chemical study and at the same time characterizing similar regions morphologically with the electron microscope.

Submitted on March 31, 1965


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