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The Journal of Cell Biology, Vol 27, 53-66, Copyright © 1965 by Rockefeller University Press

ARTICLE

THE DEMONSTRATION OF THE SUCCINIC DEHYDROGENASE SYSTEM IN BACILLUS SUBTILIS USING TETRANITRO-BLUE TETRAZOLIUM COMBINED WITH TECHNIQUES OF ELECTRON MICROSCOPY

Albert W. Sedar 1 and Ronald M. Burde 1

1 From the Daniel Baugh Institute of Anatomy, Jefferson Medical College, Philadelphia, Pennsylvania.

Dr. Burde's present address is the Department of Opthalmology, Washington University, St. Louis, Missouri

Activity of the succinic dehydrogenase system was studied in Bacillus subtilis utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evidenced by deposition of TNBT-formazan, was found on membranous organelles associated with the cytoplasmic membrane and septal plasma membrane, the nuclear area, and the plasma membrane. Flagella, sim190 A in diameter, with thorn-like projections protruded through the cell wall. Tangential-oblique sections of the cell wall showed many pores sim220 A in diameter with a center-to-center spacing of sim450 A.

Submitted on March 15, 1965


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