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The Journal of Cell Biology, Vol 27, 451-457, Copyright © 1965 by Rockefeller University Press

ARTICLE

NUCLEAR DNA AND CYTOPLASMIC DNA FROM TISSUES OF HIGHER PLANTS

Yasuo Hotta 1, Alix Bassel 1, and Herbert Stern 1

1 From the Department of Botany, University of Illinois, Urbana, Illinois.

The authors' present address is Department of Biology, University of California, San Diego, California

Young wheat roots were labeled with 32P-inorganic phosphate. Following the labeling period, roots were homogenized in a sucrose medium and fractionated into nuclei, cytoplasmic particles (including proplastids and mitochondria), and a soluble fraction containing most of the microsomes. DNA prepared from the particles had a higher buoyant density than that from the nuclei and showed a marked loss in total label if the roots were exposed to non-radioactive medium for 48 hours prior to fractionation of the cells.

Submitted on September 16, 1964


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