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The Journal of Cell Biology, Vol 28, 443-448, Copyright © 1966 by Rockefeller University Press

ARTICLE

FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS

Lester Packer 1, Park S. Nobel 1, Elizabeth L. Gross 1, and Howard C. Mel 1

1 From the Department of Physiology and the Donner Laboratory, University of California, Berkeley

A separation of spinach chloroplasts in vitro into fractions according to size (volume) and activity (light-dependent shrinkage and NADP reduction) has been achieved by stable-flow free boundary sedimentation-electrophoresis. The salient features of this chloroplast study are: (a) separation is achieved within 30 min; (b) only small density gradients are required, thus minimizing osmotic effects; (c) the fractions are collected continuously, with size fractionation being evidenced; and (d) particles are separated into fractions of higher and lower activities as compared with the control population.

Submitted on September 14, 1965


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