ARTICLE

RADIATION-STIMULATED DNA SYNTHESIS IN CULTURED MAMMALIAN CELLS

R. E. Rasmussen ¹ and R. B. Painter ¹

¹ From the Exobiology Division, Ames Research Center, National Aeronautics and Space Administration, Moffett Field, California.

Dr. Painter's present address is the Laboratory of Radiobiology, University of California Medical Center, San Francisco

A type of DNA synthesis in mammalian cells that is stimulated by ultraviolet light has been studied by means of radioautography and density gradient centrifugation. The characteristics of this synthesis are: (a) it is not semiconservative; (b) it is enhanced by the presence of 5-bromodeoxyuridine in the DNA molecule; (c) the degree of stimulation is dose dependent; (d) there is less variability in the rate of incorporation of H³-thymidine during this synthesis than during normal DNA synthesis; (e) it occurs in cells that are not in the normal DNA synthesis phase (G₁ and G₂ cells). This kind of synthesis has been found in cultured cell lines from five different species; however, in some strains, the presence of bromouracil in the DNA is required before it can be demonstrated by radioautography.

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This article has been cited by other articles:

• Widrow, R. J., Hansen, R. S., Kawame, H., Gartler, S. M., Laird, C. D. (1998). Very late DNA replication in the human cell cycle. Proc. Natl. Acad. Sci. USA 95: 11246-11250 [Abstract] [Full Text]  
• Benedict, W. F., Karon, M. (1971). Chromatid Breakage: Cytosine Arabinoside-Induced Lesions Inhibited by Ultraviolet Irradiation. Science 171: 680-682 [Abstract]  
• Epstein, J. H., Fukuyama, K., Reed, W. B., Epstein, W. L. (1970). Defect in DNA Synthesis in Skin of Patients with Xeroderma Pigmentosum Demonstrated in vivo. Science 168: 1477-1478 [Abstract]  

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