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The Journal of Cell Biology, Vol 29, 461-473, Copyright © 1966 by Rockefeller University Press

ARTICLE

AFFINITY OF ANIMAL CELL NUCLEOLI FOR NORMAL SERUM : Preliminary Characterization of Serum and Cell Components



John C. Maisel 1 and Ralph I. Lytle 1

1 From the United States Naval Medical Research Unit No. 4, United States Naval Hospital, Great Lakes, Illinois.

Dr. Maisel's present address is the Department of Pathology, University of Colorado Medical Center, Denver

Nucleoli of animal cells cultured in vitro are modified by a component of "nonimmune" animal serum. Modified nucleoli bind fluorescein-conjugated nonimmune serum proteins, as shown by calcium ion-dependent fluorescence. Analysis of serum indicates that the nucleolar-binding component is a globulin, with an electrophoretic mobility in the same region as the slow alpha-1 component in pH 8.6 Veronal buffer. The component has a low sedimentation constant (2.4S), and appears to contain glycoprotein with relatively high sialic acid content (8.5%); the latter moiety may be essential to reaction with nucleoli. The nucleolar component reacting with this alpha globulin fraction appears to be a histonelike basic protein. Primary cultures of animal cells have been supported for 1 wk through attachment, spreading, and outgrowth from colonies to confluent monolayers in medium containing a nucleolar-reactive serum fraction as the only protein supplement.

Submitted on July 30, 1965


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