J. Biophys. and Biochem. Cytol., Vol 3, 625-630, Copyright © 1957 by Rockefeller University Press
BRIEF NOTES
Cytoplasmic Incorporation of a Ribonucleic Acid Precursor in Amoeba proteus
Walter Plaut 1 and
Ronald C. Rustad 1
1 (From the Department of Botany, University of Wisconsin, Madison, and the Department of Zoology, University of California, Berkeley.)
The question of RNA synthesis in enucleate cytoplasm of Amoeba has been approached experimentally by incubating enucleate amoebae in a labelled RNA precursor and determining the incorporation into RNA autoradiographically. The results indicate that there is a cytoplasmic incorporation mechanism which can operate in the absence of the nucleus. A comparison is made between Acetabularia and Amoeba with respect to the origins of cytoplasmic RNA. It is concluded that the existing data are consistent with the assumption that some cytoplasmic RNA is of nuclear origin in both organisms.
Submitted on April 11, 1957
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