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J. Biophys. and Biochem. Cytol., Vol 3, 913-922, Copyright © 1957 by Rockefeller University Press

ARTICLE

STUDIES ON THE BIOSYNTHESIS OF COLLAGEN : II. THE CONVERSION OF 14C-L-PROLINE TO 14C-HYDROXYPROLINE BY FOWL OSTEOBLASTS IN TISSUE CULTURE



R. H. Smith Ph.D.1 and Sylvia Fitton Jackson Ph.D.1

1 From Medical Research Council, Biophysics Research Unit, Wheatstone Laboratory, King's College, London

1. Fowl osteoblasts grown in bulk tissue cultures in the presence of 14C-(L)-proline incorporated this amino acid into peptide linkage. A significant amount of the incorporated radioactivity was found in the hydroxyproline, glutamic acid, and aspartic acid fractions of the cultures.

2. The rate of formation of protein-bound 14C-hydroxyproline from 14C-(L)-proline was maximal in cultures grown for 15 hours and fell exponentially with the increasing age of the cultures.

3. 14C-(L)-glutamic acid was incorporated by the osteoblast cultures, but no significant amount was converted to hydroxyproline.

 Submitted on February 1, 1957


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