BIOGENESIS OF ENDOPLASMIC RETICULUM MEMBRANES: I. Structural and Chemical Differentiation in Developing Rat Hepatocyte

doi:10.1083/jcb.30.1.73

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ARTICLE

BIOGENESIS OF ENDOPLASMIC RETICULUM MEMBRANES : I. Structural and Chemical Differentiation in Developing Rat Hepatocyte

Gustav Dallner ¹, Philip Siekevitz ¹, and George E. Palade ¹

¹ From The Rockefeller University, New York.

Dr. Dallner's present address is the Department of Pathology, Karolinska Institutet, Stockholm, Sweden

The development of the endoplasmic reticulum of rat hepatocyteswas studied during a period of rapid cell differentiation, i.e.,from 3 days before to 8 days after birth. Before birth, theER increases in volume, remaining predominantly rough surfaced;after birth, the increase continues but affects mainly the smooth-surfacedpart of the system. These changes are reflected in variationsof the RNA/protein and PLP/protein ratios of microsomal fractions:the first decreases, while the second increases, with age. Theanalysis of microsomal membranes and of microsomal lipids indicatesthat the PLP/protein ratio, the distribution of phospholipids,and the rate of P³² incorporation into these phospholipids showlittle variation over the period examined and are comparableto values found in adult liver. Fatty acid composition of totalphosphatides undergoes, however, drastic changes after birth.During the period of rapid ER development in vivo incorporationof leucine-C¹⁴ and glycerol-C¹⁴ into the proteins and lipidsof microsomal membranes is higher in the rough-than in the smooth-surfacedmicrosomes, for the first hours after the injection of the label;later on ( $sim$ 10 hr) the situation is reversed. These results stronglysuggest that new membrane is synthesized in the rough ER andsubsequently transferred to the smooth ER.

Submitted on January 17, 1966

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