ARTICLE

ISOLATION OF METAPHASE CHROMOSOMES FROM HELA CELLS

Joel A. Huberman ¹ and Giuseppe Attardi ¹

¹ From the Division of Biology, California Institute of Technology, Pasadena

The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes contain about 2.0 mg of acid-soluble protein, 2.7 mg of acid-insoluble protein and 0.66 mg of RNA for each milligram of DNA. The RNA bound to the isolated chromosomes consists mainly of ribosomal RNA, but there is also a significant amount of 45S RNA.

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This article has been cited by other articles:

• Hanson, C. V., Hearst, J. E. (1974). Bulk Isolation of Metaphase Chromosomes from an In Vitro Cell Line of Drosophila melanogaster. Cold Spring Harb Symp Quant Biol 38: 341-345 [Abstract]  
• Bonner, J., Dahmus, M. E., Fambrough, D., Huang, R.-c. C., Marushige, K., Tuan, D. Y. H. (1968). The Biology of Isolated Chromatin: Chromosomes, biologically active in the test tube, provide a powerful tool for the study of gene action.. Science 159: 47-56 [Abstract]  

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