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The Journal of Cell Biology, Vol 38, 426-436, Copyright © 1968 by Rockefeller University Press

ARTICLE

DNA ORGANIZATION IN THE MATURE SPERM OF SEVERAL ORTHOPTERA BY THE METHOD OF POLARIZED FLUORESCENCE MICROSCOPY

James W. MacInnes 1 and Robert B. Uretz 1

1 From the Department of Biophysics, The University of Chicago, Chicago, Illinois 60637.

Dr. MacInnes' present address is the Institute for Developmental Biology, University of Colorado, Boulder, Colorado 80302

Mature sperm of Acheta domesticus, Acheta assimilis, Nemobius fasciatus, Nemobius confusus, Melanoplus femur-rubrum, Romalea microptera, Scudderia curvicauda, and Ceuthophilus nigricans were examined for DNA configuration by means of polarized fluorescence microscopy. In all cases, the results suggest that the DNA lies in unsupercoiled array, predominantly parallel to the elongate sperm head axes. Detailed calculations of the factors influencing the degree of polarization of the fluorescent emission from supercoiled DNA are contained in an appendix.

Submitted on September 14, 1967
Revised on April 17, 1968


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