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J. Biophys. and Biochem. Cytol., Vol 4, 727-730, Copyright © 1958 by Rockefeller University Press

ARTICLE

Staining of Tissue Sections for Electron Microscopy with Heavy Metals : II. Application of Solutions Containing Lead and Barium



Michael L. Watson Ph.D.1

1 (From the Departments of Pathology and Radiation Biology, University of Rochester School of Medicine and Dentistry, Rochester, New York)

Descriptions of three heavy metal stains and methods of application to tissue sections for electron microscopy are presented. Lead hydroxide stains rather selectively two types of particles in liver: those associated with the endoplasmic reticulum and containing ribonucleic acid and other somewhat larger particles. Barium hydroxide emphasizes certain bodies within vesicles of the Golgi region of hepatic cells. Alkalized lead acetate is useful as a general stain, as are also lead and barium hydroxides.

Submitted on August 1, 1958


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