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J. Biophys. and Biochem. Cytol., Vol 4, 761-764, Copyright © 1958 by Rockefeller University Press

ARTICLE

A Cellophane-Strip Technique for Culturing Tissue in Multipurpose Culture Chambers

George G. Rose M.D.1, C. M. Pomerat Ph.D.1, T. O. Shindler M.D.1, and J. B. Trunnell M.D.1

1 (From the Departments of Biology and Medicine, The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston; Tissue Culture Laboratory, The University of Texas Medical Branch, Galveston; Tissue Culture Laboratory, Hermann Hospital, Houston)

A new technique for the cultivation of living tissues in the multipurpose culture chamber is described. This procedure employs strips of cellophane as the agent for anchoring tissue explants to the coverslip walls of the chamber and disposes of the time-honored plasma-clot technique. The primary advance embodied in this procedure lies in the fact that cells emigrating from so-cultured explants manifest themselves in a highly differentiated manner comparable to the cells of origin, whereas the outgrowth from the same types of tissue in plasma clots results in a more undifferentiated type of growth. Comparisons of outgrowths from embryonic thyroid, bone, and muscle (chicken) are photographically documented, and attention is called to certain cytochemical methods which further corroborate the differentiated quality obtained with the cellophane-strip technique.

Submitted on June 19, 1958


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