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The Journal of Cell Biology, Vol 40, 120-135, Copyright © 1969 by Rockefeller University Press

ARTICLE

THE MITOTIC APPARATUS IN FUNGI, CERATOCYSTIS FAGACEARUM AND FUSARIUM OXYSPORUM

James R. Aist 1

1 From the Department of Plant Pathology, the University of Arkansas, Fayetteville, Arkansas 72701. The author's present address is the Department of Plant Pathology, the University of Wisconsin, Madison, Wisconsin 53706.

Vegetative nuclei of fungi Ceratocystis fagacearum and Fusarium oxysporum were studied both in the living condition with phase-contrast microscopy and after fixation and staining by HCl-Giemsa, aceto-orcein, and acid fuchsin techniques. Nucleoli, chromosomes, centrioles, spindles, and nuclear envelopes were seen in living hyphae of both fungi. The entire division process occurred within an intact nuclear envelope. Spindles were produced between separating daughter centrioles. At metaphase the chromosomes became attached to the spindle at different points. In F. oxysporum the metaphase chromosomes were clear enough to allow counts to be made, and longitudinal splitting of the chromosomes into chromatids was observed. Anaphase was characterized in both fungi by separation of chromosomes to poles established by the centrioles, and in F. oxysporum anaphase separation of chromosomes was observed in vivo. Continued elongation of the spindles further separated the daughter nuclei. Maturing daughter nuclei of both fungi were quite motile; and in C. fagacearum the centriole preceded the bulk of the nucleus during migration. The above observations on living cells were corroborated by observations on fixed and stained material.

Submitted on May 13, 1968
Revised on August 12, 1968


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