ARTICLE

ESTABLISHMENT OF A CLONAL STRAIN OF HEPATOMA CELLS WHICH SECRETE ALBUMIN

U. Ingrid Richardson ¹, Armen H. Tashjian Jr. ¹, and Lawrence Levine ¹

¹ From the Pharmacology Department, Harvard School of Dental Medicine and the Department of Pharmacology, Harvard Medical School, Boston, Massachusetts 02115; and the Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154

A clonal strain of epithelial cells (designated MH₁C₁) has been established from the transplantable Morris hepatoma No. 7795. The cells have maintained distinctive morphology throughout more than 20 subcultures (split 1:5) at 2- to 4-week intervals in supplemented Ham's F 10 medium. They contain many highly refractile, round, cytoplasmic bodies which stain bright red with Oil Red O. The population doubling time was 2 wk when the clonal strain was first established. It has gradually decreased to 1 wk. The cells synthesize rat serum albumin and secrete it into the culture medium as determined immunologically by microcomplement fixation and double diffusion. Albumin secretion (3–6 µg albumin/mg cell nitrogen/24 hr) occurs throughout the logarithmic phase of cell proliferation and has not diminished during serial propagation since the strain was initiated 15 months ago.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Tobin, K. A. R., Steineger, H. H., Alberti, S., Spydevold, O., Auwerx, J., Gustafsson, J.-A., Nebb, H. I. (2000). Cross-Talk between Fatty Acid and Cholesterol Metabolism Mediated by Liver X Receptor-{alpha}. Mol. Endocrinol. 14: 741-752 [Abstract] [Full Text]  
• Bayly, A., French, N., Dive, C, Roberts, R. (1993). Non-genotoxic hepatocarcinogenesis in vitro: the FaO hepatoma line responds to peroxisome proliferators and retains the ability to undergo apoptosis. J. Cell Sci. 104: 307-315 [Abstract]  
• Rugstad, H. E., Robinson, S. H., Yannoni, C., Tashjian, A. H. Jr. (1970). Transfer of Bilirubin Uridine Diphosphate-Glucuronyltransferase to Enzyme-Deficient Rats. Science 170: 553-555 [Abstract]  
• Krawitt, E. L., Baril, E. F., Becker, J. E., Potter, V. R. (1970). Amino Acid Transport in Hepatoma Cell Cultures during Tyrosine Aminotransferase Induction. Science 169: 294-296 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents