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The Journal of Cell Biology, Vol 43, 229-236, Copyright © 1969 by Rockefeller University Press

ARTICLE

REPLICATION OF NUCLEOLUS-ASSOCIATED DNA DURING "G2 PHASE" IN PHYSARUM POLYCEPHALUM

Edmund Guttes 1 and Sophie Guttes 1

1 From the Department of Biology, Loyola University, Chicago, Illinois 60626

In the myxomycete, Physarum polycephalum, the bulk of nuclear DNA replication occurs during a period of a few hours immediately following upon mitosis. During the remainder of the intermitotic period, incorporation of thymidine-3H continues at a low rate in the region of the nucleolus (radioautographs). A few nuclei incorporated thymidine-3H into the extranucleolar chromatin at a high rate at all times of the intermitotic period. These nuclei were exceptionally large and they frequently contained several small nucleoli of different sizes rather than the one, central nucleolus which is characteristic of a normal interphase nucleus.

Submitted on April 10, 1969
Revised on June 18, 1969


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