ARTICLE

HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS : A Biochemical and Fine Structural Study

M. N. Berry ¹ and D. S. Friend ¹

¹ From the Division of Clinical Pathology and the Department of Pathology, University of California School of Medicine, San Francisco, California 94122

A new technique employing continuous recirculating perfusion of the rat liver in situ, shaking of the liver in buffer in vitro, and filtration of the tissue through nylon mesh, results in the conversion of about 50% of the liver into intact, isolated parenchymal cells. The perfusion media consist of: (a) calcium-free Hanks' solution containing 0.05% collagenase and 0.10% hyaluronidase, and (b) magnesium and calcium-free Hanks' solution containing 2 mM ethylenediaminetetraacetate. Biochemical and morphologic studies indicate that the isolated cells are viable. They respire in a medium containing calcium ions, synthesize glucose from lactate, are impermeable to inulin, do not stain with trypan blue, and retain their structural integrity. Electron microscopy of biopsies taken during and after perfusion reveals that desmosomes are quickly cleaved. Hemidesmosome-containing areas of the cell membrane invaginate and appear to pinch off and migrate centrally. Tight and gap junctions, however, persist on the intact, isolated cells, retaining small segments of cytoplasm from formerly apposing parenchymal cells. Cells which do not retain tight and gap junctions display swelling of Golgi vacuoles and vacuoles in the peripheral cytoplasm. Cytoplasmic vacuolization in a small percentage of cells and potassium loss are the only indications of cell injury detected. By other parameters measured, the isolated cells are comparable to normal hepatic parenchymal cells in situ in appearance and function.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Kruger, P., Daneshfar, R., Eckert, G. P., Klein, J., Volmer, D. A., Bahr, U., Muller, W. E., Karas, M., Schubert-Zsilavecz, M., Abdel-Tawab, M. (2008). Metabolism of Boswellic Acids in Vitro and in Vivo. Drug Metab. Dispos. 36: 1135-1142 [Abstract] [Full Text]  
• Mansouri, R. M., Bauge, E., Staels, B., Gervois, P. (2008). Systemic and Distal Repercussions of Liver-Specific Peroxisome Proliferator-Activated Receptor-{alpha} Control of the Acute-Phase Response. Endocrinology 149: 3215-3223 [Abstract] [Full Text]  
• Carre, N., Cauzac, M., Girard, J., Burnol, A.-F. (2008). Dual Effect of the Adapter Growth Factor Receptor-Bound Protein 14 (Grb14) on Insulin Action in Primary Hepatocytes. Endocrinology 149: 3109-3117 [Abstract] [Full Text]  
• Katchman, H., Tal, O., Eventov-Friedman, S., Shezen, E., Aronovich, A., Tchorsh, D., Cohen, S., Shtabsky, A., Hecht, G., Dekel, B., Freud, E., Reisner, Y. (2008). Embryonic Porcine Liver as a Source for Transplantation: Advantage of Intact Liver Implants over Isolated Hepatoblasts in Overcoming Homeostatic Inhibition by the Quiescent Host Liver. Stem Cells 26: 1347-1355 [Abstract] [Full Text]  
• Venkataraman, K., Lee, Y.-M., Michaud, J., Thangada, S., Ai, Y., Bonkovsky, H. L., Parikh, N. S., Habrukowich, C., Hla, T. (2008). Vascular Endothelium As a Contributor of Plasma Sphingosine 1-Phosphate. Circ. Res. 102: 669-676 [Abstract] [Full Text]  
• Zhang, D., Raghavan, N., Chen, S.-Y., Zhang, H., Quan, M., Lecureux, L., Patrone, L. M., Lam, P. Y. S., Bonacorsi, S. J., Knabb, R. M., Skiles, G. L., He, K. (2008). Reductive Isoxazole Ring Opening of the Anticoagulant Razaxaban Is the Major Metabolic Clearance Pathway in Rats and Dogs. Drug Metab. Dispos. 36: 303-315 [Abstract] [Full Text]  
• Madsen, C. S., Janovitz, E., Zhang, R., Van Nguyen-Tran, , Ryan, C. S., Yin, X., Monshizadegan, H., Chang, M., D'Arienzo, C., Scheer, S., Setters, R., Search, D., Chen, X., Zhuang, S., Kunselman, L., Peters, A., Harrity, T., Apedo, A., Huang, C., Cuff, C. A., Kowala, M. C., Blanar, M. A., Sun, C.-q., Robl, J. A., Stein, P. D. (2008). The Guinea Pig as a Preclinical Model for Demonstrating the Efficacy and Safety of Statins. J. Pharmacol. Exp. Ther. 324: 576-586 [Abstract] [Full Text]  
• Brown, H. S., Chadwick, A., Houston, J. B. (2007). Use of Isolated Hepatocyte Preparations for Cytochrome P450 Inhibition Studies: Comparison with Microsomes for Ki Determination. Drug Metab. Dispos. 35: 2119-2126 [Abstract] [Full Text]  
• Hallifax, D., Houston, J. B. (2007). Saturable Uptake of Lipophilic Amine Drugs into Isolated Hepatocytes: Mechanisms and Consequences for Quantitative Clearance Prediction. Drug Metab. Dispos. 35: 1325-1332 [Abstract] [Full Text]  
• Pagliassotti, M. J., Wei, Y., Wang, D. (2007). Insulin Protects Liver Cells from Saturated Fatty Acid-Induced Apoptosis via Inhibition of c-Jun NH2 Terminal Kinase Activity. Endocrinology 148: 3338-3345 [Abstract] [Full Text]  
• Stock, P., Monga, D., Tan, X., Micsenyi, A., Loizos, N., Monga, S. P.S. (2007). Platelet-derived growth factor receptor-{alpha}: a novel therapeutic target in human hepatocellular cancer. Molecular Cancer Therapeutics 6: 1932-1941 [Abstract] [Full Text]  
• Erve, J. C. L., Vashishtha, S. C., DeMaio, W., Talaat, R. E. (2007). Metabolism of Prazosin in Rat, Dog, and Human Liver Microsomes and Cryopreserved Rat and Human Hepatocytes and Characterization of Metabolites by Liquid Chromatography/Tandem Mass Spectrometry. Drug Metab. Dispos. 35: 908-916 [Abstract] [Full Text]  
• Maenz, C., Chang, S.-F., Iwanski, A., Bruns, M. (2007). Entry of Duck Hepatitis B Virus into Primary Duck Liver and Kidney Cells after Discovery of a Fusogenic Region within the Large Surface Protein. J. Virol. 81: 5014-5023 [Abstract] [Full Text]  
• Gatica, A., Aguilera, M. C., Contador, D., Loyola, G., Pinto, C. O., Amigo, L., Tichauer, J. E., Zanlungo, S., Bronfman, M. (2007). P450 CYP2C epoxygenase and CYP4A {omega}-hydroxylase mediate ciprofibrate-induced PPAR{alpha}-dependent peroxisomal proliferation. J. Lipid Res. 48: 924-934 [Abstract] [Full Text]  
• Guo, Y., Jolly, R. A., Halstead, B. W., Baker, T. K., Stutz, J. P., Huffman, M., Calley, J. N., West, A., Gao, H., Searfoss, G. H., Li, S., Irizarry, A. R., Qian, H.-r., Stevens, J. L., Ryan, T. P. (2007). Underlying Mechanisms of Pharmacology and Toxicity of a Novel PPAR Agonist Revealed Using Rodent and Canine Hepatocytes. Toxicol Sci 96: 294-309 [Abstract] [Full Text]  
• Azzout-Marniche, D., Gaudichon, C., Blouet, C., Bos, C., Mathe, V., Huneau, J.-F., Tome, D. (2007). Liver glyconeogenesis: a pathway to cope with postprandial amino acid excess in high-protein fed rats?. Am. J. Physiol. Regul. Integr. Comp. Physiol. 292: R1400-R1407 [Abstract] [Full Text]  
• Bailly-Maitre, B., Bard-Chapeau, E., Luciano, F., Droin, N., Bruey, J.-M., Faustin, B., Kress, C., Zapata, J. M., Reed, J. C. (2007). Mice Lacking bi-1 Gene Show Accelerated Liver Regeneration. Cancer Res. 67: 1442-1450 [Abstract] [Full Text]  
• Zhang, D., Wang, L., Raghavan, N., Zhang, H., Li, W., Cheng, P. T., Yao, M., Zhang, L., Zhu, M., Bonacorsi, S., Yeola, S., Mitroka, J., Hariharan, N., Hosagrahara, V., Chandrasena, G., Shyu, W. C., Humphreys, W. G. (2007). Comparative Metabolism of Radiolabeled Muraglitazar in Animals and Humans by Quantitative and Qualitative Metabolite Profiling. Drug Metab. Dispos. 35: 150-167 [Abstract] [Full Text]  
• Rencurel, F., Foretz, M., Kaufmann, M. R., Stroka, D., Looser, R., Leclerc, I., da Silva Xavier, G., Rutter, G. A., Viollet, B., Meyer, U. A. (2006). Stimulation of AMP-Activated Protein Kinase Is Essential for the Induction of Drug Metabolizing Enzymes by Phenobarbital in Human and Mouse Liver. Mol. Pharmacol. 70: 1925-1934 [Abstract] [Full Text]  
• Hallifax, D., Houston, J. B. (2006). Uptake and Intracellular Binding of Lipophilic Amine Drugs by Isolated Rat Hepatocytes and Implications for Prediction of in Vivo Metabolic Clearance. Drug Metab. Dispos. 34: 1829-1836 [Abstract] [Full Text]  
• Pan, C. Q., Buxton, J. M., Yung, S. L., Tom, I., Yang, L., Chen, H., MacDougall, M., Bell, A., Claus, T. H., Clairmont, K. B., Whelan, J. P. (2006). Design of a Long Acting Peptide Functioning as Both a Glucagon-like Peptide-1 Receptor Agonist and a Glucagon Receptor Antagonist. J. Biol. Chem. 281: 12506-12515 [Abstract] [Full Text]  
• Guinzberg, R., Cortes, D., Diaz-Cruz, A., Riveros-Rosas, H., Villalobos-Molina, R., Pina, E. (2006). Inosine released after hypoxia activates hepatic glucose liberation through A3 adenosine receptors. Am. J. Physiol. Endocrinol. Metab. 290: E940-E951 [Abstract] [Full Text]  
• Simon, F. R., Iwahashi, M., Hu, L.-J., Qadri, I., Arias, I. M., Ortiz, D., Dahl, R., Sutherland, E. (2006). Hormonal regulation of hepatic multidrug resistance-associated protein 2 (Abcc2) primarily involves the pattern of growth hormone secretion. Am. J. Physiol. Gastrointest. Liver Physiol. 290: G595-G608 [Abstract] [Full Text]  
• Costet, P., Cariou, B., Lambert, G., Lalanne, F., Lardeux, B., Jarnoux, A.-L., Grefhorst, A., Staels, B., Krempf, M. (2006). Hepatic PCSK9 Expression Is Regulated by Nutritional Status via Insulin and Sterol Regulatory Element-binding Protein 1c. J. Biol. Chem. 281: 6211-6218 [Abstract] [Full Text]  
• Franco, M. C., Arciuch, V. G. A., Peralta, J. G., Galli, S., Levisman, D., Lopez, L. M., Romorini, L., Poderoso, J. J., Carreras, M. C. (2006). Hypothyroid Phenotype Is Contributed by Mitochondrial Complex I Inactivation Due to Translocated Neuronal Nitric-oxide Synthase. J. Biol. Chem. 281: 4779-4786 [Abstract] [Full Text]  
• López-Lluch, G., Hunt, N., Jones, B., Zhu, M., Jamieson, H., Hilmer, S., Cascajo, M. V., Allard, J., Ingram, D. K., Navas, P., de Cabo, R. (2006). Calorie restriction induces mitochondrial biogenesis and bioenergetic efficiency. Proc. Natl. Acad. Sci. USA 103: 1768-1773 [Abstract] [Full Text]  
• Wang, D., Wei, Y., Pagliassotti, M. J. (2006). Saturated Fatty Acids Promote Endoplasmic Reticulum Stress and Liver Injury in Rats with Hepatic Steatosis. Endocrinology 147: 943-951 [Abstract] [Full Text]  
• Wang, D., Wei, Y., Schmoll, D., Maclean, K. N., Pagliassotti, M. J. (2006). Endoplasmic Reticulum Stress Increases Glucose-6-Phosphatase and Glucose Cycling in Liver Cells. Endocrinology 147: 350-358 [Abstract] [Full Text]  
• Cassuto, H., Kochan, K., Chakravarty, K., Cohen, H., Blum, B., Olswang, Y., Hakimi, P., Xu, C., Massillon, D., Hanson, R. W., Reshef, L. (2005). Glucocorticoids Regulate Transcription of the Gene for Phosphoenolpyruvate Carboxykinase in the Liver via an Extended Glucocorticoid Regulatory Unit. J. Biol. Chem. 280: 33873-33884 [Abstract] [Full Text]  
• Garcia-Rubio, L., Matas, P., Miguez, M.-P. (2005). Protective effect of melatonin on paraquat-induced cytotoxicity in isolated rat hepatocytes. Hum Exp Toxicol 24: 475-480 [Abstract]  
• Tovar, A. R., Torre-Villalvazo, I., Ochoa, M., Elias, A. L., Ortiz, V., Aguilar-Salinas, C. A., Torres, N. (2005). Soy protein reduces hepatic lipotoxicity in hyperinsulinemic obese Zucker fa/fa rats. J. Lipid Res. 46: 1823-1832 [Abstract] [Full Text]  
• Duran-Sandoval, D., Cariou, B., Percevault, F., Hennuyer, N., Grefhorst, A., van Dijk, T. H., Gonzalez, F. J., Fruchart, J.-C., Kuipers, F., Staels, B. (2005). The Farnesoid X Receptor Modulates Hepatic Carbohydrate Metabolism during the Fasting-Refeeding Transition. J. Biol. Chem. 280: 29971-29979 [Abstract] [Full Text]  
• Wei, Y., Wang, D., Pagliassotti, M. J. (2005). Fructose Selectively Modulates c-jun N-Terminal Kinase Activity and Insulin Signaling in Rat Primary Hepatocytes. J. Nutr. 135: 1642-1646 [Abstract] [Full Text]  
• Aleman, G., Ortiz, V., Langley, E., Tovar, A. R., Torres, N. (2005). Regulation by glucagon of the rat histidase gene promoter in cultured rat hepatocytes and human hepatoblastoma cells. Am. J. Physiol. Endocrinol. Metab. 289: E172-E179 [Abstract] [Full Text]  
• Erion, M. D., van Poelje, P. D., Dang, Q., Kasibhatla, S. R., Potter, S. C., Reddy, M. R., Reddy, K. R., Jiang, T., Lipscomb, W. N. (2005). MB06322 (CS-917): A potent and selective inhibitor of fructose 1,6-bisphosphatase for controlling gluconeogenesis in type 2 diabetes. Proc. Natl. Acad. Sci. USA 102: 7970-7975 [Abstract] [Full Text]  
• Erion, M. D., van Poelje, P. D., MacKenna, D. A., Colby, T. J., Montag, A. C., Fujitaki, J. M., Linemeyer, D. L., Bullough, D. A. (2005). Liver-Targeted Drug Delivery Using HepDirect Prodrugs. J. Pharmacol. Exp. Ther. 312: 554-560 [Abstract] [Full Text]  
• Griffin, S. J., Houston, J. B. (2005). PREDICTION OF IN VITRO INTRINSIC CLEARANCE FROM HEPATOCYTES: COMPARISON OF SUSPENSIONS AND MONOLAYER CULTURES. Drug Metab. Dispos. 33: 115-120 [Abstract] [Full Text]  
• Borgognone, M., Perez, L. M., Basiglio, C. L., Ochoa, J. E., Roma, M. G. (2005). Signaling Modulation of Bile Salt-Induced Necrosis in Isolated Rat Hepatocytes. Toxicol Sci 83: 114-125 [Abstract] [Full Text]  
• Ishii, S., IIzuka, K., Miller, B. C., Uyeda, K. (2004). Carbohydrate response element binding protein directly promotes lipogenic enzyme gene transcription. Proc. Natl. Acad. Sci. USA 101: 15597-15602 [Abstract] [Full Text]  
• Wei, Y., Pagliassotti, M. J. (2004). Hepatospecific effects of fructose on c-jun NH2-terminal kinase: implications for hepatic insulin resistance. Am. J. Physiol. Endocrinol. Metab. 287: E926-E933 [Abstract] [Full Text]  
• Simon, F. R., Fortune, J., Iwahashi, M., Qadri, I., Sutherland, E. (2004). Multihormonal regulation of hepatic sinusoidal Ntcp gene expression. Am. J. Physiol. Gastrointest. Liver Physiol. 287: G782-G794 [Abstract] [Full Text]  
• Pfandler, M.-A. S., Hochli, M., Inderbitzin, D., Meier, P. J., Stieger, B. (2004). Small hepatocytes in culture develop polarized transporter expression and differentiation. J. Cell Sci. 117: 4077-4087 [Abstract] [Full Text]  
• Chen, G., Liang, G., Ou, J., Goldstein, J. L., Brown, M. S. (2004). Central role for liver X receptor in insulin-mediated activation of Srebp-1c transcription and stimulation of fatty acid synthesis in liver. Proc. Natl. Acad. Sci. USA 101: 11245-11250 [Abstract] [Full Text]  
• Wada, W., Maeshima, A., Zhang, Y.-Q., Hasegawa, Y., Kuwano, H., Kojima, I. (2004). Assessment of the function of the {beta}C-subunit of activin in cultured hepatocytes. Am. J. Physiol. Endocrinol. Metab. 287: E247-E254 [Abstract] [Full Text]  
• Dentin, R., Pegorier, J.-P., Benhamed, F., Foufelle, F., Ferre, P., Fauveau, V., Magnuson, M. A., Girard, J., Postic, C. (2004). Hepatic Glucokinase Is Required for the Synergistic Action of ChREBP and SREBP-1c on Glycolytic and Lipogenic Gene Expression. J. Biol. Chem. 279: 20314-20326 [Abstract] [Full Text]  
• Griffin, S. J., Houston, J. B. (2004). COMPARISON OF FRESH AND CRYOPRESERVED RAT HEPATOCYTE SUSPENSIONS FOR THE PREDICTION OF IN VITRO INTRINSIC CLEARANCE. Drug Metab. Dispos. 32: 552-558 [Abstract] [Full Text]  
• Zhang, Y.-L., Hernandez-Ono, A., Ko, C., Yasunaga, K., Huang, L.-S., Ginsberg, H. N. (2004). Regulation of Hepatic Apolipoprotein B-lipoprotein Assembly and Secretion by the Availability of Fatty Acids: I. DIFFERENTIAL RESPONSE TO THE DELIVERY OF FATTY ACIDS VIA ALBUMIN OR REMNANT-LIKE EMULSION PARTICLES. J. Biol. Chem. 279: 19362-19374 [Abstract] [Full Text]  
• Chakravarty, K., Wu, S.-Y., Chiang, C.-M., Samols, D., Hanson, R. W. (2004). SREBP-1c and Sp1 Interact to Regulate Transcription of the Gene for Phosphoenolpyruvate Carboxykinase (GTP) in the Liver. J. Biol. Chem. 279: 15385-15395 [Abstract] [Full Text]  
• Wu, C.-C., Lii, C.-K., Tsai, S.-J., Sheen, L.-Y. (2004). Diallyl Trisulfide Modulates Cell Viability and the Antioxidation and Detoxification Systems of Rat Primary Hepatocytes. J. Nutr. 134: 724-728 [Abstract] [Full Text]  
• Zhang, Y., Castellani, L. W., Sinal, C. J., Gonzalez, F. J., Edwards, P. A. (2004). Peroxisome proliferator-activated receptor-{gamma} coactivator 1{alpha} (PGC-1{alpha}) regulates triglyceride metabolism by activation of the nuclear receptor FXR. Genes Dev. 18: 157-169 [Abstract] [Full Text]  
• Xu, Q., Sun, X., Qiu, Y., Zhang, H., Ding, Y. (2004). The Optimal Hepatocyte Density for a Hollow-Fiber Bioartificial Liver. Annals of Clinical & Laboratory Science 34: 87-93 [Abstract] [Full Text]  
• Klohn, P.-C., Soriano, M. E., Irwin, W., Penzo, D., Scorrano, L., Bitsch, A., Neumann, H.-G., Bernardi, P. (2003). Early resistance to cell death and to onset of the mitochondrial permeability transition during hepatocarcinogenesis with 2-acetylaminofluorene. Proc. Natl. Acad. Sci. USA 100: 10014-10019 [Abstract] [Full Text]  
• Grav, H. J., Tronstad, K. J., Gudbrandsen, O. A., Berge, K., Fladmark, K. E., Martinsen, T. C., Waldum, H., Wergedahl, H., Berge, R. K. (2003). Changed Energy State and Increased Mitochondrial {beta}-Oxidation Rate in Liver of Rats Associated with Lowered Proton Electrochemical Potential and Stimulated Uncoupling Protein 2 (UCP-2) Expression: EVIDENCE FOR PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR-{alpha} INDEPENDENT INDUCTION OF UCP-2 EXPRESSION. J. Biol. Chem. 278: 30525-30533 [Abstract] [Full Text]  
• Herzer, K., Falk, C. S., Encke, J., Eichhorst, S. T., Ulsenheimer, A., Seliger, B., Krammer, P. H. (2003). Upregulation of Major Histocompatibility Complex Class I on Liver Cells by Hepatitis C Virus Core Protein via p53 and TAP1 Impairs Natural Killer Cell Cytotoxicity. J. Virol. 77: 8299-8309 [Abstract] [Full Text]  
• Xu, Q., Yu, D., Qiu, Y., Zhang, H., Ding, Y. (2003). Function of a New Internal Bioartificial Liver: An In Vitro Study. Annals of Clinical & Laboratory Science 33: 306-312 [Abstract] [Full Text]  
• TUMA, P. L., HUBBARD, A. L. (2003). Transcytosis: Crossing Cellular Barriers. Physiol. Rev. 83: 871-932 [Abstract] [Full Text]  
• Boess, F., Kamber, M., Romer, S., Gasser, R., Muller, D., Albertini, S., Suter, L. (2003). Gene Expression in Two Hepatic Cell Lines, Cultured Primary Hepatocytes, and Liver Slices Compared to the in Vivo Liver Gene Expression in Rats: Possible Implications for Toxicogenomics Use of in Vitro Systems. Toxicol Sci 73: 386-402 [Abstract] [Full Text]  
• Yabe, D., Komuro, R., Liang, G., Goldstein, J. L., Brown, M. S. (2003). Liver-specific mRNA for Insig-2 down-regulated by insulin: Implications for fatty acid synthesis. Proc. Natl. Acad. Sci. USA 100: 3155-3160 [Abstract] [Full Text]  
• Mardones, P., Pilon, A., Bouly, M., Duran, D., Nishimoto, T., Arai, H., Kozarsky, K. F., Altayo, M., Miquel, J. F., Luc, G., Clavey, V., Staels, B., Rigotti, A. (2003). Fibrates Down-regulate Hepatic Scavenger Receptor Class B Type I Protein Expression in Mice. J. Biol. Chem. 278: 7884-7890 [Abstract] [Full Text]  
• Bedu, E., Chainier, F., Sibille, B., Meister, R., Dallevet, G., Garin, D., Duchamp, C. (2002). Increased lipogenesis in isolated hepatocytes from cold-acclimated ducklings. Am. J. Physiol. Regul. Integr. Comp. Physiol. 283: R1245-R1253 [Abstract] [Full Text]  
• Commerford, S. R., Ferniza, J. B., Bizeau, M. E., Thresher, J. S., Willis, W. T., Pagliassotti, M. J. (2002). Diets enriched in sucrose or fat increase gluconeogenesis and G-6-Pase but not basal glucose production in rats. Am. J. Physiol. Endocrinol. Metab. 283: E545-E555 [Abstract] [Full Text]  
• Li, D., Zimmerman, T. L., Thevananther, S., Lee, H.-Y., Kurie, J. M., Karpen, S. J. (2002). Interleukin-1beta -mediated Suppression of RXR:RAR Transactivation of the Ntcp Promoter Is JNK-dependent. J. Biol. Chem. 277: 31416-31422 [Abstract] [Full Text]  
• Matsumoto, M., Ogawa, W., Teshigawara, K., Inoue, H., Miyake, K., Sakaue, H., Kasuga, M. (2002). Role of the Insulin Receptor Substrate 1 and Phosphatidylinositol 3-Kinase Signaling Pathway in Insulin-Induced Expression of Sterol Regulatory Element Binding Protein 1c and Glucokinase Genes in Rat Hepatocytes. Diabetes 51: 1672-1680 [Abstract] [Full Text]  
• Gonzalez, H. E., Eugenin, E. A., Garces, G., Solis, N., Pizarro, M., Accatino, L., Saez, J. C. (2002). Regulation of hepatic connexins in cholestasis: possible involvement of Kupffer cells and inflammatory mediators. Am. J. Physiol. Gastrointest. Liver Physiol. 282: G991-G1001 [Abstract] [Full Text]  
• Liu, L., Yeh, Y.-Y. (2002). S-Alk(en)yl Cysteines of Garlic Inhibit Cholesterol Synthesis by Deactivating HMG-CoA Reductase in Cultured Rat Hepatocytes. J. Nutr. 132: 1129-1134 [Abstract] [Full Text]  
• Groneberg, D. A., Grosse-Siestrup, C., Fischer, A. (2002). In Vitro Models to Study Hepatotoxicity. Toxicol Pathol 30: 394-399 [Abstract]  
• Ross, K. L., Eisenstein, R. S. (2002). Iron Deficiency Decreases Mitochondrial Aconitase Abundance and Citrate Concentration without Affecting Tricarboxylic Acid Cycle Capacity in Rat Liver. J. Nutr. 132: 643-651 [Abstract] [Full Text]  
• Tobin, K. A. R., Ulven, S. M., Schuster, G. U., Steineger, H. H., Andresen, S. M., Gustafsson, J.-A., Nebb, H. I. (2002). Liver X Receptors as Insulin-mediating Factors in Fatty Acid and Cholesterol Biosynthesis. J. Biol. Chem. 277: 10691-10697 [Abstract] [Full Text]  
• Robles-Flores, M., Rendon-Huerta, E., Gonzalez-Aguilar, H., Mendoza-Hernandez, G., Islas, S., Mendoza, V., Ponce-Castaneda, M. V., Gonzalez-Mariscal, L., Lopez-Casillas, F. (2002). p32 (gC1qBP) Is a General Protein Kinase C (PKC)-binding Protein. INTERACTION AND CELLULAR LOCALIZATION OF P32-PKC COMPLEXES IN RAT HEPATOCYTES. J. Biol. Chem. 277: 5247-5255 [Abstract] [Full Text]  
• Mitchell, C., Mallet, V. O., Guidotti, J. E., Goulenok, C., Kahn, A., Gilgenkrantz, H. (2002). Liver Repopulation by Bcl-xL Transgenic Hepatocytes. Am. J. Pathol. 160: 31-35 [Abstract] [Full Text]  
• Armstrong, M. B., Towle, H. C. (2001). Polyunsaturated fatty acids stimulate hepatic UCP-2 expression via a PPARalpha -mediated pathway. Am. J. Physiol. Endocrinol. Metab. 281: E1197-E1204 [Abstract] [Full Text]  
• Kanamaru, C., Yasuda, H., Takeda, M., Ueda, N., Suzuki, J., Tsuchida, T., Mashima, H., Ohnishi, H., Fujita, T. (2001). Smad7 Is Induced by Norepinephrine and Protects Rat Hepatocytes from Activin A-induced Growth Inhibition. J. Biol. Chem. 276: 45636-45641 [Abstract] [Full Text]  
• Nogueira, V., Rigoulet, M., Piquet, M.-A., Devin, A., Fontaine, E., Leverve, X. M. (2001). Mitochondrial Respiratory Chain Adjustment to Cellular Energy Demand. J. Biol. Chem. 276: 46104-46110 [Abstract] [Full Text]  
• Bizeau, M. E., Thresher, J. S., Pagliassotti, M. J. (2001). Sucrose diets increase glucose-6-phosphatase and glucose release and decrease glucokinase in hepatocytes. J. Appl. Physiol. 91: 2041-2046 [Abstract] [Full Text]  
• Fiedler, J., Simon, F. R., Iwahashi, M., Murphy, R. C. (2001). Effect of Peroxisome Proliferator-Activated Receptor alpha Activation on Leukotriene B4 Metabolism in Isolated Rat Hepatocytes. J. Pharmacol. Exp. Ther. 299: 691-697 [Abstract] [Full Text]  
• Bizeau, M. E., Short, C., Thresher, J. S., Commerford, S. R., Willis, W. T., Pagliassotti, M. J. (2001). Increased pyruvate flux capacities account for diet-induced increases in gluconeogenesis in vitro. Am. J. Physiol. Regul. Integr. Comp. Physiol. 281: R427-R433 [Abstract] [Full Text]  
• Viollet, B., Yaniv, M., Pontoglio, M. (2001). Embryonic but Not Postnatal Reexpression of Hepatocyte Nuclear Factor 1{alpha} (HNF1{alpha}) Can Reactivate the Silent Phenylalanine Hydroxylase Gene in HNF1{alpha}-Deficient Hepatocytes. Mol. Cell. Biol. 21: 3662-3670 [Abstract] [Full Text]  
• Párrizas, M., Maestro, M. A., Boj, S. F., Paniagua, A., Casamitjana, R., Gomis, R., Rivera, F., Ferrer, J. (2001). Hepatic Nuclear Factor 1-{alpha} Directs Nucleosomal Hyperacetylation to Its Tissue-Specific Transcriptional Targets. Mol. Cell. Biol. 21: 3234-3243 [Abstract] [Full Text]  
• Bizeau, M. E., Thresher, J. S., Pagliassotti, M. J. (2001). A high-sucrose diet increases gluconeogenic capacity in isolated periportal and perivenous rat hepatocytes. Am. J. Physiol. Endocrinol. Metab. 280: E695-E702 [Abstract] [Full Text]  
• Ledirac, N., de Sousa, G., Fontaine, F., Agouridas, C., Gugenheim, J., Lorenzon, G., Rahmani, R. (2001). Effects of Macrolide Antibiotics on CYP3A Expression in Human and Rat Hepatocytes: Interspecies Differences in Response to Troleandomycin. Drug Metab. Dispos. 28: 1391-1393 [Abstract] [Full Text]  
• Reinoso, R. F., Telfer, B. A., Brennan, B. S., Rowland, M. (2001). Uptake of Teicoplanin by Isolated Rat Hepatocytes: Comparison with in Vivo Hepatic Distribution. Drug Metab. Dispos. 29: 453-459 [Abstract] [Full Text]  
• Ireson, C., Orr, S., Jones, D. J. L., Verschoyle, R., Lim, C.-K., Luo, J.-L., Howells, L., Plummer, S., Jukes, R., Williams, M., Steward, W. P., Gescher, A. (2001). Characterization of Metabolites of the Chemopreventive Agent Curcumin in Human and Rat Hepatocytes and in the Rat in Vivo, and Evaluation of Their Ability to Inhibit Phorbol Ester-induced Prostaglandin E2 Production. Cancer Res. 61: 1058-1064 [Abstract] [Full Text]  
• Walters, H. C., Craddock, A. L., Fusegawa, H., Willingham, M. C., Dawson, P. A. (2000). Expression, transport properties, and chromosomal location of organic anion transporter subtype 3. Am. J. Physiol. Gastrointest. Liver Physiol. 279: G1188-G1200 [Abstract] [Full Text]  
• Hasmall, S. C., West, D. A., Olsen, K., Roberts, R. A. (2000). Role of hepatic non-parenchymal cells in the response of rat hepatocytes to the peroxisome proliferator nafenopin in vitro. Carcinogenesis 21: 2159-2165 [Abstract] [Full Text]  
• Muoio, D. M., Lewin, T. M., Wiedmer, P., Coleman, R. A. (2000). Acyl-CoAs are functionally channeled in liver: potential role of acyl-CoA synthetase. Am. J. Physiol. Endocrinol. Metab. 279: E1366-E1373 [Abstract] [Full Text]  
• Weglarz, T. C., Degen, J. L., Sandgren, E. P. (2000). Hepatocyte Transplantation into Diseased Mouse Liver : Kinetics of Parenchymal Repopulation and Identification of the Proliferative Capacity of Tetraploid and Octaploid Hepatocytes. Am. J. Pathol. 157: 1963-1974 [Abstract] [Full Text]  
• Eichhorst, S. T., Müller, M., Li-Weber, M., Schulze-Bergkamen, H., Angel, P., Krammer, P. H. (2000). A Novel AP-1 Element in the CD95 Ligand Promoter Is Required for Induction of Apoptosis in Hepatocellular Carcinoma Cells upon Treatment with Anticancer Drugs. Mol. Cell. Biol. 20: 7826-7837 [Abstract] [Full Text]  
• Torra, I. P., Tsibulsky, V., Delaunay, F., Saladin, R., Laudet, V., Fruchart, J.-C., Kosykh, V., Staels, B. (2000). Circadian and Glucocorticoid Regulation of Rev-erb{alpha} Expression in Liver. Endocrinology 141: 3799-3806 [Abstract] [Full Text]  
• Mitchell, C., Mignon, A., Guidotti, J. E., Besnard, S., Fabre, M., Duverger, N., Parlier, D., Tedgui, A., Kahn, A., Gilgenkrantz, H. (2000). Therapeutic liver repopulation in a mouse model of hypercholesterolemia. Hum Mol Genet 9: 1597-1602 [Abstract] [Full Text]  
• Hasmall, S. C., Roberts, R. A. (2000). The Nongenotoxic Hepatocarcinogens Diethylhexylphthalate and Methylclofenapate Induce DNA Synthesis Preferentially in Octoploid Rat Hepatocytes. Toxicol Pathol 28: 503-509 [Abstract]  
• Tobin, K. A. R., Steineger, H. H., Alberti, S., Spydevold, O., Auwerx, J., Gustafsson, J.-A., Nebb, H. I. (2000). Cross-Talk between Fatty Acid and Cholesterol Metabolism Mediated by Liver X Receptor-{alpha}. Mol. Endocrinol. 14: 741-752 [Abstract] [Full Text]  
• Padron, D., Bizeau, M. E., Hazel, J. R. (2000). Is fluid-phase endocytosis conserved in hepatocytes of species acclimated and adapted to different temperatures?. Am. J. Physiol. Regul. Integr. Comp. Physiol. 278: R529-R536 [Abstract] [Full Text]  
• El-Mir, M.-Y., Nogueira, V., Fontaine, E., Averet, N., Rigoulet, M., Leverve, X. (2000). Dimethylbiguanide Inhibits Cell Respiration via an Indirect Effect Targeted on the Respiratory Chain Complex I. J. Biol. Chem. 275: 223-228 [Abstract] [Full Text]  
• Burelle, Y., Fillipi, C., Peronnet, F., Leverve, X. (2000). Mechanisms of increased gluconeogenesis from alanine in rat isolated hepatocytes after endurance training. Am. J. Physiol. Endocrinol. Metab. 278: E35-E42 [Abstract] [Full Text]  
• Guha, C., Sharma, A., Gupta, S., Alfieri, A., Gorla, G. R., Gagandeep, S., Sokhi, R., Roy-Chowdhury, N., Tanaka, K. E., Vikram, B., Roy-Chowdhury, J. (1999). Amelioration of Radiation-induced Liver Damage in Partially Hepatectomized Rats by Hepatocyte Transplantation. Cancer Res. 59: 5871-5874 [Abstract] [Full Text]  
• Shimomura, I., Bashmakov, Y., Ikemoto, S., Horton, J. D., Brown, M. S., Goldstein, J. L. (1999). Insulin selectively increases SREBP-1c mRNA in the livers of rats with streptozotocin-induced diabetes. Proc. Natl. Acad. Sci. USA 96: 13656-13661 [Abstract] [Full Text]  
• Kassie, F., Pool-Zobel, B., Parzefall, W., Knasmuller, S. (1999). Genotoxic effects of benzyl isothiocyanate, a natural chemopreventive agent. Mutagenesis 14: 595-604 [Abstract] [Full Text]  
• Titos, E., Chiang, N., Serhan, C. N., Romano, M., Gaya, J., Pueyo, G., Claria, J. (1999). Hepatocytes are a rich source of novel aspirin-triggered 15-epi-lipoxin A4. Am. J. Physiol. Cell Physiol. 277: C870-C877 [Abstract] [Full Text]  
• Foretz, M., Guichard, C., Ferre, P., Foufelle, F. (1999). Sterol regulatory element binding protein-1c is a major mediator of insulin action on the hepatic expression of glucokinase and lipogenesis-related genes. Proc. Natl. Acad. Sci. USA 96: 12737-12742 [Abstract] [Full Text]  
• Berk, P. D., Zhou, S.-L., Kiang, C.-L., Stump, D. D., Fan, X., Bradbury, M. W. (1999). Selective Up-regulation of Fatty Acid Uptake by Adipocytes Characterizes Both Genetic and Diet-induced Obesity in Rodents. J. Biol. Chem. 274: 28626-28631 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents