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The Journal of Cell Biology, Vol 44, 513-529, Copyright © 1970 by Rockefeller University Press

ARTICLE

HEMOGLOBIN UPTAKE BY RAT HEPATOCYTES AND ITS BREAKDOWN WITHIN LYSOSOMES

Sidney Goldfischer 1, Alex B. Novikoff 1, Arline Albala 1, and Luis Biempica 1

1 From the Departments of Pathology and Medicine, Albert Einstein College of Medicine of Yeshiva University, New York, 10461

The peroxidatic activity of hemoglobin permitted visualization of its uptake by rat hepatocytes by means of the Graham-Karnovsky 3,3'-diaminobenzidine (DAB) procedure. Lysosomes were visualized by their acid phosphatase, ß-glucuronidase, and glucosaminidase activities. When large doses of rat, cow, or human hemoglobin are intravenously injected, or when hemoglobinemia is induced by injection of distilled water, DAB-positive hemoglobin is engulfed by pinocytosis. Pinocytotic vacuoles become digestive vacuoles ("phagolysosomes") by fusion with lysosomes of the dense body type that have moved from their pericanalicular position. By 16–24 hr after even massive amounts of hemoglobin (400 mg/100 g), the protein is barely demonstrable in hepatocytes. At the lowest doses of injected hemoglobin (15 mg/100 g body weight), DAB-positive vacuoles are demonstrable only in the Kupffer cells.

Submitted on February 14, 1969
Revised on October 7, 1969


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