ARTICLE

CEREBELLAR GRANULE CELLS IN VITRO : A Light and Electron Microscope Study

Fredrick J. Seil ¹ and Robert M. Herndon ¹

¹ From the Division of Neurology, Stanford University School of Medicine, Stanford, California 94305, and the Department of Neurology, Veterans Administration Hospital, Palo Alto, California 94304

The behavior of granule cells in mature cerebellar cultures derived from newborn mice was studied by light and electron microscopy. Many granule cells remained in the explants as an external granular layer. These cells were differentiated, as evidenced by formation of bundles of parallel fibers and by development of synapses between granule cell axons and Purkinje cell branchlet spines, and between Golgi cell axons and granule cell dendrites. Although the over-all architecture of the cerebellar explants after 18–33 days in vitro was similar to that of the newborn mouse, the evident differentiation of the granule cells suggested that interneuronal relationships resemble those of the mature cerebellum in vivo.

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This article has been cited by other articles:

• Lapham, L. W., Markesbery, W. R. (1971). Human Fetal Cerebellar Cortex: Organization and Maturation of Cells in vitro. Science 173: 829-832 [Abstract]  

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