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The Journal of Cell Biology, Vol 45, 235-245, Copyright © 1970 by Rockefeller University Press

ARTICLE

ELECTRON MICROSCOPY OF THE AMMONIACAL SILVER REACTION FOR HISTONES IN THE ERYTHROPOIETIC CELLS OF THE CHICK

Edith K. MacRae 1 and Gerald D. Meetz 1

1 From the Department of Anatomy, University of Illinois at the Medical Center, Chicago, Illinois 60612.

Dr. Meetz's present address is the Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80302

The product of the postformalin ammoniacal silver reaction, which has been claimed to distinguish lysine-rich from arginine-rich histones with the light microscope on the basis of a color difference, was examined in developing erythroid cells of chick bone marrow with the electron microscope. Stem cells and early erythroblasts exhibit no, or little, ammoniacal silver reaction product, while small basophilic erythroblasts, polychromatophilic erythrocytes, and reticulocytes exhibit an increasing amount of reaction product as maturation proceeds. The reaction product is in the form of discrete electron-opaque particles associated with heterochromatin. The ammoniacal silver reaction in the erythroid cell series is interpreted as reflecting either the accumulation of newly synthesized arginine-rich histones or changes in the availability of reactive sites in preformed histones.

Submitted on July 9, 1969
Revised on December 18, 1969


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