ARTICLE

THE SYNTHESIS AND TURNOVER OF RAT LIVER PEROXISOMES : III. The Size Distribution of Peroxisomes and the Incorporation of New Catalase

Brian Poole ¹, Tokuhiko Higashi ¹, and Christian de Duve ¹

¹ From The Rockefeller University, New York 10021.

Dr. Higashi's present address is the School of Pharmacy, Showa University, 1-5-8 Hatano-Dai, Shinawaga-Ku, Tokyo, Japan

Rat liver peroxisomes have been separated according to size by zonal sedimentation. A method is described for calculating the size of the particles from their final position in the gradient. Peroxisomes seem biochemically homogeneous throughout their size distribution. 3 hr after injection of tritiated leucine, the specific radioactivity of catalase is the same in peroxisomes of different sizes, and it remains so for up to 1 wk after administration of the precursor. This observation rules out the possibility that peroxisomes have an extended period of independent growth. If individual particles maintain an independent existence, they must be formed very rapidly. The other possible explanation is that peroxisomes exchange material within the liver cell.

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• Passreiter, M., Anton, M., Lay, D., Frank, R., Harter, C., Wieland, F. T., Gorgas, K., Just, W. W. (1998). Peroxisome Biogenesis: Involvement of ARF and Coatomer. JCB 141: 373-383 [Abstract] [Full Text]  
• Hill, P., Walton, P. (1995). Import of microinjected proteins bearing the SKL peroxisomal targeting sequence into the peroxisomes of a human fibroblast cell line: evidence that virtually all peroxisomes are import-competent. J. Cell Sci. 108: 1469-1476 [Abstract]  
• Duve, C (1975). Exploring cells with a centrifuge. Science 189: 186-194  

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