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The Journal of Cell Biology, Vol 45, 493-508, Copyright © 1970 by Rockefeller University Press

ARTICLE

MEMBRANE MODIFICATIONS IN NUTRITIONALLY INDUCED FILAMENTOUS ESCHERICHIA COLI B

George Weinbaum 1, Donald A. Fischman 1, and Shinichi Okuda 1

1 From the Department of Biochemistry, Research Laboratories, Albert Einstein Medical Center, Philadelphia, Pennsylvania 19141, and The Department of Biology, University of Chicago, Chicago, Illinois 60637

Nutritionally induced filamentous cell forms of Escherichia coli B were examined for their morphological and biochemical lesions. The filamentous forms showed no significant alteration in total DNA concentration, RNA synthesis, ability to form ß-galactosidase in response to isopropylthiogalactoside, or insensitivity to actinomycin D as compared to the normal cell form. The filamentous cells showed a marked decrease in the ability to incorporate N-acetylglucosamine-UL-14C into a phenol-soluble glycoprotein fraction relative to the normal cell form or relative to strain E-26 of E. coli grown in the filament-inducing medium. The filaments yielded an envelope-specific phenol-soluble protein fraction markedly reduced in or lacking three proteins as determined by acrylamide gel electrophoresis. Amino acid analysis, and chemical and enzymatic treatments of the envelope-specific phenol-soluble proteins showed striking differences between the fractions obtained from normal and filamentous cells. Electron microscope studies of divalent cation-induced aggregates of the envelope proteins showed different aggregation patterns dependent upon the cell form yielding the protein fraction.

Submitted on June 16, 1969
Revised on December 1, 1969


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