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The Journal of Cell Biology, Vol 47, 384-394, Copyright © 1970 by Rockefeller University Press

ARTICLE

PURIFICATION OF INTACT MICROTUBULES FROM BRAIN

Joel B. Kirkpatrick 1, Lyon Hyams 1, Virginia L. Thomas 1, and Peter M. Howley 1

1 From the Department of Pharmacology, Rutgers Medical School, New Brunswick, New Jersey 08903

Hundred-fold purification of intact microtubules from homogenates of rat brain is reported. The success of purification depends on stabilizing the microtubule structure by the combined effects of hexylene glycol, acidic Ph, and low temperature. A practical, negative stain, electron microscopic assay is used to study purity and stability of microtubule fractions. The purified fractions show a major band which migrates like purified tubulin in the SDS gel electrophoresis system.

Submitted on February 3, 1970
Revised on June 29, 1970


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