ARTICLE

MICRONUCLEAR RIBONUCLEIC ACID IN TETRAHYMENA PYRIFORMIS

Kuruganti G. Murti ¹ and David M. Prescott ¹

¹ From the Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80302

The presence of RNA in the micronucleus of Tetrahymena pyriformis was detected by electron microscope radioautography after incubation with tritiated precursors. The specificity of RNA labeling was shown by ribonuclease digestion. The period of appearance of labeled RNA in the micronucleus is approximately coincident with the DNA synthesis period for the micronucleus. Pulse-chase experiments showed that the micronuclear RNA disappears during the interphase period. The experiments do not distinguish whether the micronuclear RNA is synthesized in situ or acquired by migration from the macronucleus. In either case it is notable that the appearance of labeled RNA is detected in the micronucleus only during the micronuclear S phase.

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This article has been cited by other articles:

• Gorovsky, M. A., Glover, C., Johmann, C. A., Keevert, J. B., Mathis, D. J., Samuelson, M. (1978). Histones and Chromatin Structure in Tetrahymena Macro- and Micronuclei. Cold Spring Harb Symp Quant Biol 42: 493-503 [Abstract]  

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