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The Journal of Cell Biology, Vol 47, 619-630, Copyright © 1970 by Rockefeller University Press

ARTICLE

STUDIES ON NUCLEAR STRUCTURE AND FUNCTION IN TETRAHYMENA PYRIFORMIS : II. Isolation of Macro- and Micronuclei



Martin A. Gorovsky 1

1 From the Whitman Laboratory, the Department of Biology, The University of Chicago, Chicago, Illinois 60637.

Dr. Gorovsky's present address is the Department of Biology, The University of Rochester, Rochester, New York 14627

This report describes a rapid, efficient method for isolating macronuclei from Tetrahymena. The macronuclear fraction contains only small amounts of micronuclear material and little detectable whole cell or cytoplasmic contamination. A method is also described for preparing a "micronuclear fraction" which contains 20–40 micronuclei for every macronucleus present. Electron microscope observations indicate that the ultrastructure of the nuclei in the macronuclear fraction closely resembles that of nuclei in situ. The presence of ribosomes on the outer membrane of micronuclei and of pores in the micronuclear envelope is also described.

Submitted on March 4, 1970
Revised on July 28, 1970


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