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The Journal of Cell Biology, Vol 48, 15-28, Copyright © 1971 by Rockefeller University Press

ARTICLE

FERRITIN IN THE FUNGUS PHYCOMYCES

Charles N. David 1 and Kenneth Easterbrook 1

1 From the Division of Biology, California Institute of Technology, Pasadena, California 91109.

Dr. David's present address is the Max-Planck Institut für Virusforschung, 74 Tübingen, West Germany. Dr. Easterbrook's present address is the Department of Microbiology, Dalhousie University, Halifax, Nova Scotia, Canada

The iron-protein ferritin has been purified from mycelium, sporangiophores, and spores of the fungus Phycomyces blakesleeanus. It has a protein-to-iron ratio of 5, a sedimentation coefficient of 55S, a buoyant density in CsCl of 1.82 g/cm3, and the characteristic morphology of ferritin in the electron microscope. Apoferritin prepared from Phycomyces ferritin has a sedimentation coefficient of 18S and consists of subunits of molecular weight 25,000. In the cytoplasm of Phycomyces, ferritin is located on the surface of lipid droplets (0.5–2.0 µ in diameter) where it forms crystalline monolayers which are conspicuous in electron micrographs of sporangiophore thin-sections. Ferritin is found in all developmental stages of Phycomyces but is concentrated in spores. The level of ferritin iron is regulated by the iron level in the growth medium, a 50-fold increase occurring on iron-supplemented medium.

Submitted on November 14, 1968
Revised on July 13, 1970


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