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The Journal of Cell Biology, Vol 48, 225-234, Copyright © 1971 by Rockefeller University Press

ARTICLE

THE EXPRESSION OF DIFFERENTIATION BY CHICK EMBRYO THYROID IN CELL CULTURE : II. Modification of Phenotype in Monolayer Culture by Different Media



Brian S. Spooner 1 and S. Robert Hilfer 1

1 From the Department of Biology, Temple University, Philadelphia, Pennsylvania 19122.

Dr. Spooner's present address is the Department of Biological Sciences, Stanford University, Stanford, California 94305.

Previous results with thyroid secretory cells in monolayer culture seem contradictory with respect to phenotypic stability of this cell type. On the one hand, in "minimal" medium the cells lose structural and functional specializations which can be returned only by three-dimensional growth in organ culture upon addition of fibroblasts derived from the thyroid capsule. On the other hand, in "rich" medium used for cloning, cytoarchitecture and function remain unaltered in either mass or clonal cultures. The apparent discrepancy has been resolved by plating cell suspensions in both media and changing to the alternate medium once the cells have become established. It has been shown that a number of characteristics, including hormone levels, are reversed each time such a change in medium is made. These modulations are discussed in terms of the normal variations in structure and function of the gland in vivo.

Submitted on January 29, 1970
Revised on July 31, 1970


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