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The Journal of Cell Biology, Vol 48, 314-323, Copyright © 1971 by Rockefeller University Press

ARTICLE

THE DISTRIBUTION OF INORGANIC CATIONS IN MOUSE TESTIS : Electron Microscope and Microprobe Analysis



Abraham L. Kierszenbaum 1, Cesar M. Libanati 1, and Carlos J. Tandler 1

1 From the Centro de Investigaciones sobre Reproducción, Facultad de Medicina, and the Comisión Nacional de Energía Atómica, and the Instituto de Anatomía General y Embriología, Facultad de Medicina, Buenos Aires, Argentine

For localization of pyroantimonate-precipitable cations, mouse testes were fixed with a saturated aqueous solution of potassium pyroantimonate (pH about 9.2, without addition of any conventional fixative), hardened with formaldehyde, and postosmicated. A good preservation of the cell membranes and over-all cell morphology is obtained as well as a consistent and reproducible localization of the insoluble antimonate salts of magnesium, calcium, and sodium. Four sites of prominent antimonate deposits are revealed, besides a more or less uniform distribution of the precipitates. These sites are: (a) In the walls of the seminiferous tubules, localized in two concentric layers corresponding to the inner and outer layers of the tubular wall; (b) Around the blood vessels and adjacent connective tissue; (c) At the area of contact between the Sertoli cell and spermatids, where a double line of precipitate surrounds the head of the mature spermatids; and (d) In the cell nuclei, disposed between regions of the condensed chromatin. The nucleus of mature spermatids did not show any sign of antimonate precipitation. The implications of this inorganic cation distribution are discussed with relation to their anionic counterparts, their localization in other animal and plant tissues, and the possibility that those sites may represent barriers to the free passage of ions.

Submitted on May 18, 1970
Revised on July 20, 1970


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