ARTICLE

THE M BAND : Studies with Fluorescent Antibody Staining

Ellen Kundrat ¹ and Frank A. Pepe ¹

¹ From the Department of Anatomy, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19104

The M band can be extracted from fibrils suspended in 5 mM Tris buffer, pH 8.0, for 15 min. The M band is completely removed only from fibrils of sarcomere lengths greater than 2.1 µ. Extraction does not alter the fluorescent antimyosin staining pattern of the A band, thus providing strong evidence that no alteration of the structural integrity of the thick filament has occurred. Fluorescent antibody staining of the M band of unextracted fibrils can be prevented specifically by absorbing the fluorescent antibody with extracted M band material prior to staining. This verifies the specificity of the extraction procedure.

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This article has been cited by other articles:

• Offer, G. (1973). C-Protein and the Periodicity in the Thick Filaments of Vertebrate Skeletal Muscle. Cold Spring Harb Symp Quant Biol 37: 87-93 [Abstract]  

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