ARTICLE

LOCALIZATION OF THE SITES OF -AMINOBUTYRIC ACID (GABA) UPTAKE IN LOBSTER NERVE-MUSCLE PREPARATIONS

Paula M. Orkand ¹ and Edward A. Kravitz ¹

¹ From the Department of Anatomy, University of California at Los Angeles School of Medicine, Los Angeles, California 90024, and the Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115

The principal sites of -aminobutyric acid (GABA) uptake in lobster nerve-muscle preparations have been determined with radioautographic techniques after binding of the amino acid to proteins by aldehyde fixation. Semiquantitative studies showed that about 30% of the radioactive GABA taken into the tissue was bound to protein by fixation. Both light and electron micrographs showed dense accumulations of label over Schwann and connective tissue cell cytoplasm; muscle was lightly labeled, but axons and terminals were almost devoid of label. The possible role of Schwann and connective tissue cells in the inactivation of GABA released from inhibitory axons is discussed.

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