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The Journal of Cell Biology, Vol 49, 235-246, Copyright © 1971 by Rockefeller University Press

ARTICLE

FREE-FLOW ELECTROPHORETIC SEPARATION AND ELECTRICAL SURFACE PROPERTIES OF SUBCELLULAR PARTICLES FROM GUINEA PIG BRAIN

K. J. Ryan 1, H. Kalant 1, and E. Llewellyn Thomas 1

1 From the Department of Pharmacology and Institute of Biomedical Electronics, University of Toronto, Toronto, Canada

Continuous free-flow electrophoretic separation has been used to obtain relatively pure preparations of synaptosomes and synaptic vesicles from crude fractions of guinea pig brain homogenates. Measurements of the contents of protein, neuraminic acid, and bound acetylcholine; the activities of succinic dehydrogenase, adenosine triphosphatase, choline acetylase, and 5'-nucleotidase; and the uptake of 14C-labeled choline arid acetylcholine in the presence and absence of hemicholinium, all confirm the electron microscope evidence that the electrophoretic preparations are at least as pure as those obtained by ultracentrifugal methods. The electrophoretic mobility measurements have been used to calculate zeta potentials and surface charge densities for these particles.

Submitted on June 8, 1970
Revised on November 30, 1970


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