ARTICLE

ISOLATION OF NUCLEI FROM YEAST

Madhu M. Bhargava ¹ and Harlyn O. Halvorson ¹

¹ From the Laboratory of Molecular Biology and the Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

A method for isolation of nuclei from Saccharomyces cervisiae in high yield is described. The DNA/protein ratio of the isolated nuclei is 10 times higher than that of whole cells. Examination of these nuclei in phase and electron microscopes has shown them to be round bodies having a double membrane, microtubules, and a dark crescent at one end. The optimum conditions for extraction and resolution of histones of these nuclei on acrylamide gels have been investigated. The nuclei have an active RNA polymerase (E.C. 2.7.7.6) and are able to synthesize RNA in vitro. They are also readily stainable with Giemsa's, Feulgen's, and acridine orange methods.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Beckmann, J., Johnson, P., Abelson, J (1977). Cloning of yeast transfer RNA genes in Escherichia coli. Science 196: 205-208 [Abstract]  
• Cryer, D. R., Goldthwaite, C. D., Zinker, S., Lam, K.-B., Storm, E., Hirschberg, R., Blamire, J., Finkelstein, D. B., Marmur, J. (1974). Studies on Nuclear and Mitochondrial DNA of Saccharomyces cerevisiae. Cold Spring Harb Symp Quant Biol 38: 17-29 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents