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The Journal of Cell Biology, Vol 49, 450-458, Copyright © 1971 by Rockefeller University Press

ARTICLE

LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization



K. E. Kuettner 1, R. Eisenstein 1, L. W. Soble 1, and C. Arsenis 1

1 From the Departments of Orthopaedic Surgery and Biochemistry and Division of Pathology, Rush Presbyterian-St. Luke's Medical Center, and Department of Biological Chemistry, University of Illinois College of Medicine, Chicago, Illinois 60612

The localization of chick embryonic lysozyme was determined by two techniques: by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry. Both techniques indicate that, in this tissue, lysozyme is primarily extra-cellular. Cartilage lysozyme was isolated and partially characterized and found to be identical with egg white lysozyme in its immunologic and enzymatic behavior. In addition, a method for the isolation of large numbers of viable chondrocytes is described.

Submitted on May 8, 1970
Revised on December 7, 1970


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