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The Journal of Cell Biology, Vol 49, 459-467, Copyright © 1971 by Rockefeller University Press

ARTICLE

ENZYME ACTIVITIES IN CHICK EMBRYONIC CARTILAGE : Their Subcellular Distribution in Isolated Chondrocytes



C. Arsenis 1, R. Eisenstein 1, L. W. Soble 1, and K. E. Kuettner 1

1 From the Department of Biological Chemistry, University of Illinois College of Medicine, and Departments of Orthopaedic Surgery and Biochemistry and Division of Pathology, Rush Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612

Some characteristic enzymatic activities were determined in chick embryonic cartilage and compared with the analogous activities in bone and liver. Chondrocytes were isolated, broken by sonication, and subjected to subcellular fractionation to yield a nuclear pellet, the mitochondrial, lysosomal, and microsomal fractions, and the high speed supernatant solution. It was established that these fractions are characterized by enzymatic activities usually associated with similar fractions in other tissues, but with some quantitative differences. Lysozyme, a particulate-associated enzyme in other tissues, was not detected in any subcellular fraction even by the sensitive technique of microzone electrophoresis and is therefore considered to be primarily extracellular in cartilage.

Submitted on May 8, 1970
Revised on December 7, 1970


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