ARTICLE

STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS : II. Isolation and Lipid Analysis of Cell Fractions

Yoshinori Nozawa ¹ and Guy A. Thompson Jr. ¹

¹ From the Department of Botany, University of Texas, Austin, Texas 78712.

Dr. Nozawa's permanent address is Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu, Japan

A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals significant differences in lipid composition. Most noteworthy are the high concentrations of phosphonolipid and tetrahymanol in the surface membranes.

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