JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 560K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nozawa, Y.
Right arrow Articles by Thompson, G. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nozawa, Y.
Right arrow Articles by Thompson, G. A., Jr.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Cell Biology, Vol 49, 722-730, Copyright © 1971 by Rockefeller University Press

ARTICLE

STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS : III. Lipid Incorporation into Various Cellular Membranes of Logarithmic Phase Cultures



Yoshinori Nozawa 1 and Guy A. Thompson Jr. 1

1 From the Department of Botany, University of Texas, Austin, Texas 78712.

Dr. Nozawa's permanent address is Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu, Japan

When 1-14C-palmitic acid is used to pulse label logarithmic cultures of Tetrahymena pyriformis, radioactivity appears in lipids of the various membrane types at vastly differing rates. The microsomes and postmicrosomal supernatant attain a high specific radioactivity within 1 min, while the membranes enveloping the cilia require several hours to reach the microsomal level. A similar pattern is obtained when the tracer is sodium 1-14C-acetate or 8,9-3H-hexadecyl glycerol. In all fractions the phosphonolipid incorporates radioactivity from 14C-palmitate much less rapidly than do the other major phospholipids. The patterns of labeling suggest that new lipids are transported from a cytoplasmic site of synthesis to points of membrane fabrication throughout the cell.

Submitted on August 11, 1970
Revised on October 1, 1970


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents